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Samples had been then gathered and added using an equivalent quantity of cold acetonitrile. The samples have been saved at −eighty °C until Assessment. The samples have been thawed and diluted a hundred× with cell phase ahead of injection.
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Samples had been then collected and extra with the equal quantity of cold acetonitrile. The samples were being saved at −80 °C right until Investigation. The samples have been thawed and diluted 100× with cellular stage before injection.